Using Elisa and nested PCR for detection of the toxoplasmosis in milk and the influence of infection and some factors on milk composition in the Iraqi local and Shami goats

This study concluded detection of Toxoplasma gondii in milk, immunologically by using Elisa and nested PCR)nPCR (based on B1 gene, also to investigate the effect toxoplasmosis, parity, breed flock some milk composition Iraqi local Shami goats middle Iraq. A total 80 samples lactating were collected. Results this showed prevalence Toxoplasmosis was 21.25% 28.75% nPCR respectively without significant differences. The sensitivity a low (30.43%) whereas specificity high (82.45%). degree agreement estimated Kappa coefficient revealed slight (0.14) between two methods. results indicated that infected with toxoplasmosis have significantly (P<0.05) higher protein lactose percentage not for each fat, SNF pH. On other hand, parity protein, lactose, solid nonfat non-effect PH. As well as fat